Pengujian Pakis Kinca (Nephrolepis Cordifolia (L) C. Presl) Sebagai Inhibitor enzyim sitokrom sub time 2E1 Dengan Metode Doking

Rizka Aulia  Rahmadani; Elvina Astria  Agustin; Samsul Hadi

doi:10.58794/jfarm.v2i2.680

Authors

Rizka Aulia Rahmadani Universitas Lambung Mangkurat
Elvina Astria Agustin Universitas Lambung Mangkurat
Samsul Hadi Unversitas Lambung Mangkurat

DOI:

https://doi.org/10.58794/jfarm.v2i2.680

Keywords:

sitokrom P450, docking, eugenol

Abstract

CYP450 adalah enzim yang terlibat dalam monooksigenasi beragam senyawa asing dan endogen. Dari 57 enzim manusia, mungkin sepertiga bertanggung jawab metabolisme xenobiotik, termasuk obat-obatan. Penelitian ini menggunakan metode docking untuk melihat potensi senyawa yang terkandung dari Nephrolepis Cordifolia sebagai inhibitor CYP2E1. Doking yang dilakukan menggunkan senyawa yang terkandung dari Nephrolepis Cordifolia. Metode yang digunakan dalam docking adalah rigid docking menggunakan software autodock4. Analisis data yang digunakan adalah adanya ikatan hidrogen dan enrgy Gibbs. Hasil dari penelitian ini yaitu energi terendah adalah β-sitosterol dengan energi Gibbs -8.233 kcal/mol. Ikatan yang terbentuk adalah ikatan hidrofob. benzyl butyl phthalate, β-ionone, oleanolic acid terjadiny ikatan hidrofob. Myristic acid membentuk ikatan hidrogen dengan HIS 109. Eugenol membentuk ikatan hidrogen dengan THR303. Kesimpulan senyawa yang berpotensi sebagai inhibitor CYP2E1 adalah Myristic acid dan Eugenol.

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