Pakis Kinca (Nephrolepis Cordifolia (L) C. Presl) Sebagai Inhibitor Nitric Oxide Synthase

Elvina Astria Agustin; Noor Annisa  Rizkiyah; Samsul Hadi

doi:10.58794/jfarm.v2i2.679

Authors

Elvina Astria Agustin Universitas Lambung Mangkurat
Noor Annisa Rizkiyah Universitas Lambung Mangkurat
Samsul Hadi Unversitas Lambung Mangkurat

DOI:

https://doi.org/10.58794/jfarm.v2i2.679

Keywords:

doking, NOS, Oleanolic acid

Abstract

Nitric oxide (NO) adalah molekul kecil, dan dihasilkan dari asam amino L-arginine (L-Arg) oleh tiga enzim nitric oxide synthase (NOS). Kadar kecil NO didalam darah dikaitkan dengan hipertensi, impotensi, aterosklerosis, dan penyakit kardiovaskular, sedangkan kelebihan NO menyebabkan peradangan, artritis reumatoid, penyakit radang usus, diabetes tipe imun, stroke, kanker, trombosis, dan infeksi. Metode penelitian ini megggunakan metode docking dengan software Autodock vina model rigid docking. Sennyawa yang digunakan berasal dari Nephrolepis Cordifolia. Visulisasi data hasil doking menggunakan Dicovery studio. Analisis data menggunkaan kombinasi energi Gibbs dan terjadinyaikatan hirogen. Hasil diperoleh skor doking dari senyawa Nephrolepis cordifolia terhadap enzim Nitric Oxide Synthase. Energi gibs terendah diperoleh oleh senyawa β-sitosterol yaitu -9.4270 kcal/mole. Dan ikatan yang terbentuk adalah ikatan Hidrofob. Senyawa fern-9(11)-ene dan β-ionone adalah hidrofob. Oleanolic acid mampu membentuk ikatan hidrogen dengan residu Arg199 dan Met 120. Benzyl butyl phthalate membentuk ikatan hidrogen dengan CYS200 dan TRP372. Ligand native (omega-imidazolyl-decanoic acid) mampu membentuk ikatan hidrogen dengan Arg199 dan TYR 489.Kesimpulan senyawa yang berpotensi sebagai inhibitor nitric oxide synthaseadalah Oleanolic acid dan Benzyl butyl phthalate.

References

U. Förstermann and W. C. Sessa, “Nitric oxide synthases: regulation and function.,” Eur. Heart J., vol. 33, no. 7, pp. 829–37, 837a-837d, Apr. 2012, doi: 10.1093/eurheartj/ehr304.

W. K. Alderton, C. E. Cooper, and R. G. Knowles, “Nitric oxide synthases: structure, function and inhibition.,” Biochem. J., vol. 357, no. Pt 3, pp. 593–615, Aug. 2001, doi: 10.1042/0264-6021:3570593.

F. Shari and G. Ahmed, “The Level of Nitric Oxide Synthase and Nitric Oxide in Hypertensive Women,” J. Pharm. Negat. Results, vol. 13, pp. 236–239, Oct. 2022.

P. Verma, “Role of Nitric Oxide Synthase in Platelets of Breast Carcinoma,” Jul. 2022.

S. Mulani et al., “Morphological, Histological and Phytochemical Features of Nephrolepis cordifolia (L.) C. Presl,” Natl. Acad. Sci. Lett., Sep. 2023, doi: 10.1007/s40009-023-01348-2.

P. Potensi, D. Jus, R. Nefrolepis, T. Tikus, A. Rajasekaran, and S. Vellaichamy, “Evaluation of Diuretic Potential of Nephrolepis cordifolia Rhizome Juice in Wistar Rats,” Sains Malaysiana, vol. 38, pp. 57–59, Feb. 2009.

F. J. Mary, D. M. S. Kumar, E. Vijaykumar, G. Yadeshwaran, V. Yamuna, and E. Yuvalakshmi, “Nephrolepis cordifolia: A Review on the fern,” J. Univ. Shanghai Sci. Technol., vol. 23, pp. 301–305, Oct. 2021, doi: 10.51201/JUSST/21/09689.

G. Ahmed, F. Shry, H. Almashhadani, and M. Kadhim, “The Level of Nitric Oxide Synthase and Nitric Oxide in Hypertensive Women,” J. Pharm. Negat. Results, vol. 13, pp. 236–239, Sep. 2022, doi: 10.47750/pnr.2022.13.03.038.

J. Shamsara et al., “Evaluation of serum nitric oxide synthase levels in patients with coronary slow flow based on corrected TIMI frame count,” Arch. Med. Sci. - Atheroscler. Dis., vol. 8, pp. 140–145, Jan. 2024, doi: 10.5114/amsad/176659.

X.-X. Xu et al., “Neuronal nitric oxide synthase/reactive oxygen species pathway is involved in apoptosis and pyroptosis in epilepsy,” Neural Regen. Res., vol. 18, p. 1277, Jun. 2023, doi: 10.4103/1673-5374.357906.

J. Lee et al., “Neuronal nitric oxide synthase is required for erythropoietin stimulated erythropoiesis in mice,” Front. Cell Dev. Biol., vol. 11, p. 1144110, Feb. 2023, doi: 10.3389/fcell.2023.1144110.

H. Li et al., “Crystallographic and Computational Insights into Isoform-Selective Dynamics in Nitric Oxide Synthase,” Biochemistry, vol. 63, Feb. 2024, doi: 10.1021/acs.biochem.3c00601.

A. Alam, S. Smith, S. Gobalakrishnan, M. McGinn, V. Yakovlev, and C. Rabender, “Uncoupled nitric oxide synthase activity promotes colorectal cancer progression,” Front. Oncol., vol. 13, p. 1165326, Mar. 2023, doi: 10.3389/fonc.2023.1165326.

O. Mishra, R. Mishra, Q. M. Ashraf, and M. Delivoria-Papadopoulos, “Nitric oxide-mediated mechanism of neuronal nitric oxide synthase and inducible nitric oxide synthase expression during hypoxia in the cerebral cortex of newborn piglets,” Neuroscience, vol. 140, pp. 857–863, Aug. 2006, doi: 10.1016/j.neuroscience.2006.02.060.